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notch1 signaling  (MedChemExpress)


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    Structured Review

    MedChemExpress notch1 signaling
    Notch1 Signaling, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/notch1+signaling/DAPT/pm41962853-255-8-13
    Average 97 stars, based on 153 article reviews
    notch1 signaling - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Numb attenuates LPS-induced neuroinflammation via autophagic regulation of Ifi204 in microglia.
    Article Snippet: LPS stimulation was used to induce BV2 cells activation (1μg/mL LPS, Escherichia coli 055: B5, Sigma Aldrich, L6529). .. DAPT was used to inhibit the activation of Notch1 signaling (20 nM DAPT, MCE, HY-13027). .. Bafilomycin A1 was used to block autolysosome degradation (100 nM BafA1, Sangon Biotech, 88899–55-2).

    Article Title: Notch1 regulates Orai1 and Orai3 expression in breast cancer cells.
    Article Snippet: .. To investigate the functional role of Notch1 signaling, cells were treated with 50 μM recombinant Jagged1 protein (Jagged-1 (188-204), Anaspec Inc, Freemont, CA, USA) for 48 h to activate the pathway, and with the γ-secretase inhibitor DAPT (MedChem Express, Monmouth Junction, NJ, USA) to inhibit Notch1 activation for 24h, 48h, 72h or once daily for five consecutive days, depending of experimental procedures. .. Cells cultured in 100-mm Petri dishes (5 × 106 cells) were lysed in ice-cold NP-40 lysis buffer (137 mM NaCl, 20 mM Tris, 2 mM EDTA, 10% glycerol, 1% Nonidet P-40) supplemented with 1 mM sodium orthovanadate (Na3VO4), and a complete EDTA-free protease inhibitor cocktail (Reference name: COEDTAF-RO; Roche Diagnostics GmbH, AR TIC LE IN PR ES S Mannheim, Germany).

    Functional Assay:

    Article Title: Notch1 regulates Orai1 and Orai3 expression in breast cancer cells.
    Article Snippet: .. To investigate the functional role of Notch1 signaling, cells were treated with 50 μM recombinant Jagged1 protein (Jagged-1 (188-204), Anaspec Inc, Freemont, CA, USA) for 48 h to activate the pathway, and with the γ-secretase inhibitor DAPT (MedChem Express, Monmouth Junction, NJ, USA) to inhibit Notch1 activation for 24h, 48h, 72h or once daily for five consecutive days, depending of experimental procedures. .. Cells cultured in 100-mm Petri dishes (5 × 106 cells) were lysed in ice-cold NP-40 lysis buffer (137 mM NaCl, 20 mM Tris, 2 mM EDTA, 10% glycerol, 1% Nonidet P-40) supplemented with 1 mM sodium orthovanadate (Na3VO4), and a complete EDTA-free protease inhibitor cocktail (Reference name: COEDTAF-RO; Roche Diagnostics GmbH, AR TIC LE IN PR ES S Mannheim, Germany).

    Recombinant:

    Article Title: Notch1 regulates Orai1 and Orai3 expression in breast cancer cells.
    Article Snippet: .. To investigate the functional role of Notch1 signaling, cells were treated with 50 μM recombinant Jagged1 protein (Jagged-1 (188-204), Anaspec Inc, Freemont, CA, USA) for 48 h to activate the pathway, and with the γ-secretase inhibitor DAPT (MedChem Express, Monmouth Junction, NJ, USA) to inhibit Notch1 activation for 24h, 48h, 72h or once daily for five consecutive days, depending of experimental procedures. .. Cells cultured in 100-mm Petri dishes (5 × 106 cells) were lysed in ice-cold NP-40 lysis buffer (137 mM NaCl, 20 mM Tris, 2 mM EDTA, 10% glycerol, 1% Nonidet P-40) supplemented with 1 mM sodium orthovanadate (Na3VO4), and a complete EDTA-free protease inhibitor cocktail (Reference name: COEDTAF-RO; Roche Diagnostics GmbH, AR TIC LE IN PR ES S Mannheim, Germany).

    Infection:

    Article Title: MiR-4332 modulates oxidative stress, inflammasome activation through notch1 signaling during Toxoplasma gondii infection.
    Article Snippet: Toxoplasma gondii manipulates host cell pathways through complex strategies.. Oxidative stress and inflammasome activation are key to its pathogenesis, yet the mechanisms by which the parasite triggers these processes to cause host damage remain unclear.. In this study, we revealed that T. gondii drives an oxidative stressinflammation cascade culminating in cellular apoptosis.

    In Vitro:

    Article Title: Inhibition of Notch1 signaling reduces hepatocyte injury in nonalcoholic fatty liver disease via autophagy.
    Article Snippet: The human hepatocyte cell lines HepG2 were obtained from the China Center for Type Culture Collection (CCTCC) and cultured in a humidified incubator at 37 C with 5% CO2 using DMEM (supplemented with 1% penicillin and streptomycin and 10% fetal bovine serum). .. To establish the in vitro NAFLD model, HepG2 cells were cultured in 1 mM free fatty acids (FFA, containing oleic acid and palmitic acid (Sigma-Aldrich, USA) at a 2:1 vol ratio) for 24 h. To investigate the effect of the Notch1 signaling and autophagy, the cells were pretreated with Notch inhibitor N-[N-(3,5Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) (Med Chem Express, USA) or autophagy inhibitor chloroquine (CQ) (Sigma-Aldrich, USA) for 2 h and then combined treated with FFA for 24 h. ..

    Cell Culture:

    Article Title: Inhibition of Notch1 signaling reduces hepatocyte injury in nonalcoholic fatty liver disease via autophagy.
    Article Snippet: The human hepatocyte cell lines HepG2 were obtained from the China Center for Type Culture Collection (CCTCC) and cultured in a humidified incubator at 37 C with 5% CO2 using DMEM (supplemented with 1% penicillin and streptomycin and 10% fetal bovine serum). .. To establish the in vitro NAFLD model, HepG2 cells were cultured in 1 mM free fatty acids (FFA, containing oleic acid and palmitic acid (Sigma-Aldrich, USA) at a 2:1 vol ratio) for 24 h. To investigate the effect of the Notch1 signaling and autophagy, the cells were pretreated with Notch inhibitor N-[N-(3,5Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) (Med Chem Express, USA) or autophagy inhibitor chloroquine (CQ) (Sigma-Aldrich, USA) for 2 h and then combined treated with FFA for 24 h. ..



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